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Journal: iScience
Article Title: Peripheral amylin modulation rebalances brain glycolysis and Tau-Ser214 phosphorylation via cAMP-PKA signaling
doi: 10.1016/j.isci.2026.115157
Figure Lengend Snippet: Effects of circulating amylin modulation on AD-like pathology in vivo and in human amylin-treated neonatal neurons (A–D) Heat maps comparing the brain tissue levels of pT231-tau, tau, Aβ 40 , and Aβ 42 in the same mice as in , , , , and (i.e., hA ON vs. hA i−OFF mice and hA OFF vs. hA i−ON mice). (E) Aβ immunofluorescence signal for Aβ (green) of cultured primary neurons incubated with human amylin (hAmylin; 1 μM for 4 h) or under control conditions (control). MAP2 (blue) was used to identify the neurons. n = 86 neurons from 3 primary neonatal rat neuron cultures. (F) Total Aβ level secreted in the culture media by primary neurons incubated with human amylin (hAmylin; 1 μM for 2 h) or under control conditions (control). Aβ was enriched by immunoprecipitation and measured by Western blot. (G) pTau and total Tau lysates from primary neurons incubated with human amylin (hAmylin; 1 μM for 2 h) or under control conditions (control). pTau and Tau were enriched by immunoprecipitation and measured by western blot. Individual data points and mean ± s.e.m for three neuronal cultures. (H) Effect of amylin on pS214-tau in cultured murine astrocytes. Cells were incubated for 30 min under control conditions or in the presence of human amylin (15 μM) with and without the amylin receptor antagonist AC187 (10 μM) or PKA inhibitor H-89 (10 μM). pS214-tau was measured by ELISA. Individual data points and mean ± s.e.m for three cell cultures. (I) Representative images of IHC analyses of cortical slices from hA ON mice stained for amylin (top) and for total Aβ (bottom) ( n = 5 slices/mouse from n = 3 mice). Statistical analyses were performed using one-way ANOVA followed by Dunnett’s multiple-comparisons test (H) and two-tail t test (E–G). See also .
Article Snippet: Primary neuronal cells were detected with
Techniques: In Vivo, Immunofluorescence, Cell Culture, Incubation, Control, Immunoprecipitation, Western Blot, Enzyme-linked Immunosorbent Assay, Staining